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KMID : 0605620090160010005
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Journal of Korean Society of Biological Psychiatry
2009 Volume.16 No. 1 p.5 ~ p.14
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Chronic Treatment of Fluoxetine Increases Expression of NCAM140 in the Rat Hippocampus
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CHoi Mi-Ran
Chai Young-Gyu
Jung Kyoung-Hwa
Baik Seung-Youn
Kim Seok-Hyeon
Roh Sung-Won
Choi Joon-Ho
Lee Jun-Seok
Choi Ihn-Geun
Yang Byung-Hwan
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Abstract
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Objective : Most of the mechanisms reported for antidepressant drugs are the enhancement of neurite outgrowth and neuronal survival in the rat hippocampus. Neural cell adhesion molecule 140 (NCAM140) has been implicated as having a role in cell-cell adhesion, neurite outgrowth, and synaptic plasticity. In this report, we have performed to elucidate a correlation among chronic antidepressant treatments, NCAM140 expression, and activation of phosphorylated cyclicAMP responsive element binding protein (pCREB) which is a downstream molecule of NCAM140-mediated intracellular signaling pathway in the rat hippocampus.
Methods : Fluoxetine (10 mg/kg) was injected acutely (daily injection for 5 days) or chronically (daily injection for 14 days) in adult rats. RNA and protein were extracted from the rat hippocampus, respectively. Real-time RT-PCR was performed to analyze the expression pattern of NCAM140 gene and western blot analyses for the activation of the phosphorylation ratio of CREB.
Results : Chronic fluoxetine treatments increased NCAM140 expression 1.3 times higher than control in rat hippocampus. pCREB immunoreactivity in the rat hippocampus with chronic fluoxetine treatment was increased 4.0 times higher than that of control.
Conclusion : Chronic fluoxetine treatment increased NCAM140 expression and pCREB activity in the rat hippocampus. Our data suggest that NCAM140 and pCREB may participate in the clinical efficacy of antidepressants promoting the neurite outgrowth and neuronal survival.
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KEYWORD
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Fluoxetine, hippocampus, NCAM140, CREB, pCREB
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